Assessment of the added clinical value next generation sequencing of the 16S-23S rRNA region in a clinical setting (ECCMID2019)
16 april, 2019
A.M.D. Kooistra-Smid1,2, E. van Zanten1, G.J. Wisselink1, G.D. Mithoe1, R. F. de Boer1, A. Ott1, A. W. Friedrich2, R.F.J. Benus1, J.W.A. Rossen2
Certe, Department of Medical Microbiology, Groningen, The Netherlands, 2University of Groningen, University Medical Center Groningen, Department of Medical Microbiology and Infection Prevention, Groningen, The Netherlands
Serology and clinical classification in a recent case series of neurosyphilis patients (ECCMID2019)
16 april, 2019
Alewijn Ott1, Ingrid M. Daey Ouwens2,3, Aernoud Fiolet4, Peter J. Koehler5, Martin Vos6, J. Marja Oldhoff6, Willem M.A. Verhoeven2,3
1 Certe, Department of Medical Microbiology, Groningen, The Netherlands; 2 Top Clinical Centre for Neuropsychiatry, Vincent van Gogh Institute, Venray, the Netherlands; 3 Erasmus MC, Department of Psychiatry, Rotterdam,
the Netherlands; 4 UMC Utrecht, Department of Cardiology, the Netherlands; 5 Zuyderland Medical Centre, Heerlen, the Netherlands; 6 UMC Groningen, Department of Dermatology and Venereology, the Netherlands
Next generation sequencing of the 16S -23S rRNA region compared to culture and 16S rDNA Sanger sequencing for the identification of bacterial species in clinical samples
16 april, 2019
E. van Zanten1, G.J. Wisselink1, A. Ott1, R.F.J. Benus1, G.D. Mithoe1, R.F. de Boer1, A.W. Friedrich2, J.W.A. Rossen2, A.M.D. Kooistra-Smid1,2
1Certe, Department of Medical Microbiology, Groningen, The Netherlands; 2University of Groningen, University Medical Center Groningen, Department of Medical Microbiology and Infection Prevention, Groningen, The Netherlands
Selenite enrichment broth to improve the sensitivity in molecular diagnostics of Salmonella.
23 december, 2018
Auteurs
Boer MD (1), de Boer RF (2), Lameijer A (3), Sterne E (1), Skidmore B (4), Suijkerbuijk AWM (6), Heck M (6), van der Zanden AGM (1).
- Laboratory for Medical Microbiology and Public Health, LabMicTA, Hengelo.
- Certe - Laboratory for Infectious Diseases, Groningen.
- Department of Internal Medicine, ZGT, Almelo.
- Department of Public Health, Enschede.
- RIVM, Bilthoven.
De publicatie beschrijft de toegevoegde waarde van het gebruik maken van een verrijkingsstap in vloeibaar medium (seleniet), om de sensitiviteit van moleculaire diagnostiek voor detectie van Salmonella spp. in feces te verhogen.
Diagnostic stewardship: zin of onzin?!
5 november, 2018
Diagnostisch stewardship of diagnostisch stewardshipprogramma (DSP) is een trending topic in het veld van de medische microbiologie en daarbuiten. Maar waar gaat dit concept nu over, is het echt zo nieuw en hoe wordt het ingelijfd in infectiemanagement? De term ‘diagnostic stewardship’ werd gebruikt in een opiniestuk van Dik et al., waarin verschillende facetten van infectiemanagement beschreven werden, het zogeheten ‘integrated stewardship’. Wij belichten de diagnostische kant van dit model en beschrijven het concept ervan: diagnostiek als multidisciplinair geheel van opname tot ontslag.
Schijnbare thyreotoxicose door gebruik van biotine in hoge dosis. Verstoring van TSH- en T4-bepaling door biotine.
19 juli, 2018
Het is belangrijk dat clinici zich bewust zijn van de mogelijke interferentie van biotine bij immunoassays, zoals die van de schildklierfunctie, maar ook bij andere immunochemische bepalingen. Overleg met laboratoriumspecialisten bij onbegrepen uitslagen is aan te bevelen. Door volledig te vertrouwen op deze laboratoriumuitslagen ligt het gevaar van een onterechte behandeling op de loer.
Borderline QuantiFERON results and the distinction between specific responses and test variability
4 juni, 2018
QuantiFERON (QFT) results near the cut-off are subject to debate. We aimed to investigate which borderline QFT results were due to Mycobacterium tuberculosis (Mtb)-specific responses or to test variability.
Defining Multidrug Resistance of Gram-Negative Bacteria in the Dutch–German Border Region—Impact of National Guidelines
26 januari, 2018
We willen graag met z’n allen (multi)resistentie van bacteriën tegen gaan, zodat we als we later groot en sterk oud en afgetakeld zijn ook nog met antibiotica behandeld kunnen worden. Maar wanneer noem je iets nu ‘multiresistent’? Die definitie is per land verschillend en daarom hebben we gekeken naar het verschil tussen Nederland en Duitsland. Wij noemen deze bacteriën BRMO (bijzonder resistente micro-organismen) en Duitsland noemt een deel van deze bacteriën MRGN (Multiresistente gramnegative Stäbchen). Hoe is het voor medewerkers van een Infectiepreventie in de grensregio; kunnen zij deze verschillen van definities hanteren?
Gepubliceerd op: http://www.mdpi.com/2076-2607/6/1/11/htm
A multicentre verification study of the QuantiFERON®-TB Gold Plus assay
3 september, 2017
Abstract
Objectives: The aim of this verification study was to compare the QuantiFERON®-TB Gold Plus (QFT-Plus) to the QuantiFERON®-TB Gold In Tube (QFT-GIT). The new QFT-Plus test contains an extra antigen tube which, according
to the manufacturer additionally elicits a CD8+ T-cell response above the CD4+ T-cell response. We assessed the value of this tube in detecting recent latent tuberculosis infections. Methods: Between May 2015 and December 2016, 1031 subjects underwent QFT-Plus and QFT-GIT test. Overall agreement between both tests and performance for different test indications and/or immune states was assessed. A difference of>0.6 IU/mL interferon-γ release between the two antigen tubes of the QFT-Plus assay was considered a true difference and used as estimation for CD8+ T-cell response. Results: Analysis of the QuantiFERON tests resulted in an overall agreement between assays of 95%. Subjects considered to be recently exposed to tuberculosis had significantly more often a true difference in interferon-γ release compared to all other subjects (p = 0.029). Conclusion: Results of QFT-Plus are highly comparable to QFT-GIT. Although there is an indication that a true
difference in interferon-γ release between the antigen tubes is associated with recent latent tuberculosis infection, the QFT-Plus could not be used to exclude recent exposure.
Published: https://www.journals.elsevier.com/tuberculosis
Extensive colonization with carbapenemase-producing microorganisms in Romanian burn patients: infectious consequences from the Colectiv fire disaster
29 augustus, 2017
Abstract Health care of severe burn patients is highly specialized and may require international patient transfer. Burn
patients have an increased risk of developing infections. Patients that have been hospitalized in countries where carbapenemase-producing microorganisms (CPMO) are endemic
may develop infections that are difficult to treat. In addition, there is a risk on outbreaks with CPMOs in burn centers. This study underlines that burn patients may extensively be colonized with CPMOs, and it provides best practice
recommendations regarding clinical microbiology and infection control. We evaluated CPMO-carriage and wound colonization in a burn patient initially treated in Romania, and transported to the Netherlands. The sequence types and acquired
beta-lactamase genes of highly-resistant microorganisms were derived from next generation sequencing data. Next, we searched literature for reports on CPMOs in burn patients. Five different carbapenemase-producing isolates
were cultured: two unrelated OXA-48-producing Klebsiella pneumoniae isolates, OXA-23-producing Acinetobacter baumanii, OXA-48-producing Enterobacter cloacae, and NDM-1-producing Providencia stuartii. Also, multi-drug resistant
Pseudomonas aeruginosa isolates were detected. Among the sampling sites, there was high variety in CPMOs. We found 46 reports on CPMOs in burn patients. We listed the epidemiology of CPMOs by country of initial
treatment, and summarized recommendations for care of these
patients based on these reports and our study.
Published in Eur J Clin Microbiol Infect Dis